Myosin-3B and its Light Chains

Dissociation of light chains from cardiac myosin.

The substrate, ATP, protects the active site of cardiac myosin during a 10-min treatment at 37 "C and neutral pH in the absence of divalent cations; under these conditions there is an approximate 20 % dissociation of light chain C1 and 60 % loss of light chain CZ with no corresponding decrease in myosin ATPase activity. Higher temperatures, the absence of divalent cations, increased treatment t...

Detection of serum cardiac myosin light chains in acute experimental myocardial infarction: radioimmunoassay of cardiac myosin light chains.

To develop a more specific plasma test for myocardial infarction, antibodies specific for cardiac myosin light chains (CM-LC) were elicited that showed less than 3% cross-reactivity with skeletal muscle light chains. These antibodies were used to develop a radioimmunoassay for CM-LC that had a sensitivity of 20 ng (+/- 4 SD; P less than 0.001). Normal dog plasma showed no measurable concentrati...

Electrophoretic and immunological studies on the light chains of myosin.

Hybrids of Physarum myosin light chains and desensitized scallop myofibrils

The two light chains of Physarum myosin have been purified in a 1:1 ratio with a yield of 0.5-1 mg/100 g of plasmodium and a purity of 40-70%; the major contaminant is a 42,000-dalton protein. The 17,700 Mr Physarum myosin light chain (PhLC1) binds to scallop myofibrils, providing the regulatory light chains (ScRLC) have been removed. The 16,500 Mr light (PhLC2) does not bind to scallop myofibr...

Synthesis of myosin heavy and light chains in muscle cultures

The weight ratio of myosin/actin, the myosin heavy chain content as the percentage of total protein (wt/wt), and the kinds of myosin light chains were determined in (a) standard muscle cultures, (b) pure myotube cultures, and (c) fibroblast cultures. Cells for these cultures were obtained from the breast of 11-day chick embryos. Standard cultures contain, in addition to myotubes, large numbers ...